The test saw Valentino Rossi's debut on Bridgestone tyres and marked the first public appearance for the new Ducati Desmosedici GP8.
World champion Casey Stoner, fastest on day one, injured his shoulder on Wednesday and didn't ride on the final day - when Dani Pedrosa was fastest on the 2007 Honda RC212V.
Suzuki was the only factory not present in Spain, having instead chosen to develop its GSV-R at Phillip Island in Australia.
The winter test ban starts on December 1 and lasts until January 20.
1. Dani Pedrosa SPA Repsol Honda Team (M) 1min 38.562 secs
2. Nicky Hayden USA Repsol Honda Team (M) 1min 38.848 secs
3. Jorge Lorenzo SPA Fiat Yamaha Team (M) 1min 39.464 secs
4. Andrea Dovizioso ITA JiR Team Scot (M) 1min 39.747 secs
5. Shinya Nakano JPN Honda Gresini (B) 1min 40.121 secs
6. Colin Edwards USA Yamaha Tech 3 (M) 1min 40.184 secs
7. Alex de Angelis RSM Honda Gresini (B) 1min 40.185 secs
8. James Toseland GBR Yamaha Tech 3 (M) 1min 40.213 secs
9. Randy de Puniet FRA Honda LCR (M) 1min 40.398 secs
10. John Hopkins USA Kawasaki Racing Team (B) 1min 40.984 secs
11. Valentino Rossi ITA Fiat Yamaha Team (B) 1min 41.538 secs
12. Marco Melandri ITA Ducati Marlboro Team (B) 1min 41.619 secs
13. Tady Okada JPN Honda Test Rider (M) 1min 41.715 secs
14. Nicola Canepa ITA Ducati Test Rider (B) 1min 41.793 secs
15. Anthony West AUS Kawasaki Racing Team (B) 1min 42.343 secs
16. Vittoriano Guareschi ITA Ducati Test Rider (B) 1min 42.850 secs
17. Shinichi Ito JPN Ducati/Bridgestone Test Rider (B) 1min 43.001 secs
